兩種懸浮填料在ASBBR厭氧氨氧化系統(tǒng)中的性能比較與微生物解析Performance comparison and microbial analysis of two kinds of suspend
發(fā)布日期：2020-04-17 瀏覽次數(shù)：
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核心提示：兩種懸浮填料在ASBBR厭氧氨氧化系統(tǒng)中的性能比較與微生物解析Performance comparison and microbial analysis of two kinds of suspended fillers in the anammox system of ASBBRWang Jun1, Yu Deshuang1, Wang Xiaoxia1, Song Xinxin2, Li Chuanju2, Li Jia2, Lin Jia2,*, Liu Jie2,*關(guān)鍵詞：城市生活污水 厭氧
- 兩種懸浮填料在ASBBR厭氧氨氧化系統(tǒng)中的性能比較與微生物解析
- Performance comparison and microbial analysis of two kinds of suspended fillers in the anammox system of ASBBRWang Jun1, Yu Deshuang1, Wang Xiaoxia1, Song Xinxin2, Li Chuanju2, Li Jia2, Lin Jia2,*, Liu Jie2,*
- 基金項(xiàng)目：國(guó)家水體污染控制與治理科技重大專(zhuān)項(xiàng)(2017ZX07102-003); 國(guó)家自然科學(xué)基金(No. 51978348, 51778304)
- 王鈞
- 青島大學(xué)環(huán)境科學(xué)與工程學(xué)院
- 于德爽
- 青島大學(xué)環(huán)境科學(xué)與工程學(xué)院
- 王曉霞
- 青島大學(xué)環(huán)境科學(xué)與工程學(xué)院
- 摘要：選取T1聚乙烯和T2聚氨酯兩種懸浮填料投入兩套相同厭氧序批式生物膜反應(yīng)器(ASBBR) R1和R2中 ,以城市生活污水為配水基質(zhì) ,考察兩種填料在ASBBR的性能及菌群結(jié)構(gòu)特征 ,選出更適宜厭氧氨氧化菌(ANAMMOX)生長(zhǎng)的填料.結(jié)果表明 :R1和R2反應(yīng)器分別在第93 d 和73 d 成功啟動(dòng)厭氧氨氧化 ,并實(shí)現(xiàn)穩(wěn)定運(yùn)行.穩(wěn)定運(yùn)行階段R1、R2系統(tǒng)總無(wú)機(jī)氮(TIN)平均去除率分別達(dá)88.34%、87.97%.此外 ,運(yùn)行第150 d時(shí) ,測(cè)得單個(gè)T1、T2填料的ANAMMOX活性分別為5.70 mg·g-1·h-1和3.70 mg·g-1·h-1.同時(shí) ,掃描電子顯微鏡(SEM)表征結(jié)果證明 ,球狀菌在T1填料上的數(shù)量比同倍數(shù)下T2填料上多 ,且雜菌較少. 高通量測(cè)序結(jié)果顯示 ,T1、T2填料上Candidatus anammoxoglobus菌屬相對(duì)豐度分別為75.29%和38.23%.本研究表明 ,相比于T2聚氨酯填料 ,T1聚乙烯填料更適宜ANAMMOX菌的富集.
- Abstract：In order to select a more suitable kind of suspended carrier for anaerobic ammonium oxidation (ANAMMOX) bacteria, two types of carriers which named T1 polyethylene and T2 polyurethane were tested in two parallel sets of the anaerobic sequencing batch biofilm reactor (ASBBR) R1 and R2. The performance and bacterial structure on two different kinds of carrier in ASBBR were studied with municipal sewage as water distribution matrix. The experiment showed that R1 and R2 reactors successfully started on the 93rd day and the 73rd day respectively and both achieved stable operation. During the stable phase, the average TIN removal efficiency of R1 and R2 had reached up to 88.34% and 87.97%, respectively. In addition, the activity of ANAMMOX on a single T1 and T2 carrier was 5.70 mg·g-1·h-1 and 3.70 mg·g-1·h-1 on the 150th day. Meanwhile, scanning electron microscope(SEM) characterization results indicated that the number of globular bacteria on T1 carrier was more than that on T2 at the same magnification and also has fewer heterozygous bacteria. The high-throughput sequencing result demonstrated that the relative abundance of Candidatus anammoxoglobus on T1 and T2 carrier was 75.29% and 38.23%, respectively. This study concludes that T1 polyethylene carrier is more suitable for the enrichment of ANAMMOX bacteria than T2 polyurethane carrier.
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