[1]劉敏,房闊,王凱軍.AAO+流態(tài)化生物載體工藝的菌群結(jié)構分析[J].中國給水排水,2023,39(19):11-18.
LIUMin,FANGKuo,WANGKai-jun.Analysis on Microbial Community Composition of AAO+FBC/MBBR System[J].China Water & Wastewater,2023,39(19):11-18.點擊復制
AAO+流態(tài)化生物載體工藝的菌群結(jié)構分析
中國給水排水[ISSN:1000-4062/CN:12-1073/TU] 卷: 第39卷 期數(shù): 2023年第19期 頁碼: 11-18 欄目: 出版日期: 2023-10-01
Title:Analysis on Microbial Community Composition of AAO+FBC/MBBR System
作者:劉敏， 房闊， 王凱軍（清華大學環(huán)境學院 環(huán)境模擬與污染控制國家重點聯(lián)合實驗室，北京 100084）
Author(s):LIU Min， FANG Kuo， WANG Kai-jun（State Key Joint Laboratory of Environmental Simulation and Pollution Control， School of Environment， Tsinghua University， Beijing 100084， China）
關鍵詞:城市污水; 脫氮除磷; FBC/MBBR工藝; 菌群結(jié)構; 宏基因組分析
Keywords:municipal wastewater; removal of nitrogen and phosphorus; FBC/MBBR process; community composition; metagenomic analysis
摘要:為進一步提高脫氮除磷效率，某污水處理廠在好氧池投加填料，將生化段工藝升級為改進型AAO+流態(tài)化生物載體工藝（FBC/MBBR）。為考察工藝穩(wěn)定運行后的微生物菌群結(jié)構及功能，采用宏基因組測序方法對缺氧池混合液、好氧池填料和混合液樣本進行分析。結(jié)果表明，在門水平上，缺氧池混合液、好氧池填料和混合液中的優(yōu)勢類群均為變形菌門（Proteobacteria）、放線菌門（Actinobacteria）和綠彎菌門（Chloroflexi）。檢測到的聚磷菌（PAO）主要為Accumulibacter（0.81%~0.99%）、Tetrasphaera（0.35%~0.75%）、Mycobacterium（1.00%~1.54%）；在好氧池填料上檢測到較高豐度的Nitrosomonas（2.8%）和Nitrospira（2.8%）；反硝化功能菌主要包括Dechloromonas、Flavobacterium等。此外，還量化比較分析了與硝化、反硝化、厭氧氨氧化等脫氮過程相關的功能基因。
Abstract:To improve the removal efficiency of nitrogen and phosphorus, the biochemical treatment of a full-scale wastewater treatment plant was renovated into improved AAO and fluidized biological carrier process (FBC/MBBR) by adding fillers to an aerobic tank. In order to investigate the composition and function of the microbial community under stable operation, metagenomic analysis was used to further analyze the samples from anoxic tank, aerobic tank filler and aerobic tank in this wastewater treatment system. Taxonomic analysis showed that the dominant bacterial phyla were Proteobacteria, Actinobacteria and Chloroflexi. The dominant polyphosphate accumulating organism (PAO) found were Accumulibacter (0.81%-0.99%), Tetrasphaera (0.35%-0.75%) and Mycobacterium (1.00%-1.54%). The relative abundance of ammonia-oxidizing bacteria (AOB) and nitrite-oxidizing bacteria (NOB) bacteria were enriched in aerobic biofilm, and the typical AOB and NOB were Nitrosomonas (2.8%) and Nitrospira (2.8%), respectively. The denitrification functional bacteria detected mainly included Dechloromonas, Flavobacterium and so on. Otherwise, various key enzymes related to nitrification, denitrification and Anammox during nitrogen removal were annotated and quantified.
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更新日期/Last Update: 2023-10-01
