MA Jian-ye,RONG Hong-wei,WANG Jing-yin,et al.Microbial Community Composition and Membrane Fouling Performance of Anaerobic Ammonia Oxidation Membrane Bioreactor[J].China Water & Wastewater,2021,37(9):7-14.
MBR中厭氧氨氧化菌群落結(jié)構(gòu)及膜污染性能研究
- Title:
- Microbial Community Composition and Membrane Fouling Performance of Anaerobic Ammonia Oxidation Membrane Bioreactor
- 關(guān)鍵詞:
- 厭氧氨氧化; 浮游態(tài); 脫氮; 膜污染; 群落結(jié)構(gòu)
- Keywords:
- anaerobic ammonia oxidation (Anammox); planktonic state; nitrogen removal; membrane fouling; microbial community composition
摘要:- 采用浸沒式厭氧氨氧化膜生物反應(yīng)器(Amx IMBR)培養(yǎng)厭氧氨氧化菌，當(dāng)進(jìn)水氨氮與亞硝態(tài)氮濃度均為450 mg/L、氮負(fù)荷為0.6 kg/(m3·d)時(shí)，總氮去除率穩(wěn)定在86%左右，氨氮、亞硝態(tài)氮去除率分別穩(wěn)定在90%、97%以上；氨氮去除量 ∶亞硝態(tài)氮去除量 ∶硝態(tài)氮生成量基本為1 ∶1 ∶0.18。Amx IMBR的臨界通量為8.63 L/(m2·h)，這與長(zhǎng)期運(yùn)行的結(jié)果相符。膜污染的來源主要是緊密型胞外聚合物，其中蛋白質(zhì)和多糖含量分別為2.42、0.84 g/m2。運(yùn)行過程中，厭氧氨氧化菌形態(tài)從紅棕色顆粒污泥變成了浮游態(tài)污泥，是Amx IMBR最顯著的特征之一。高通量分析結(jié)果顯示，浮游態(tài)污泥中的優(yōu)勢(shì)菌群主要為擬桿菌門(Bacteroidetes，47.83%)、浮霉菌門(Planctomycetes，21.2%)、變形菌門(Proteobacteria，18.85%)，其中優(yōu)勢(shì)厭氧氨氧化菌屬為Candidatus Kuenenia，相對(duì)豐度為17.08%。
Abstract:- Anammox bacteria were cultured in an immersed anaerobic ammonia oxidation membrane bioreactor (Amx IMBR). When NH4+-N and NO2--N in the influent were both 450 mg/L and nitrogen loading rate was 0.6 kg/(m3·d),the removal rates of TN, NH4+-N and NO2--N stably reached 86%, above 90% and 97%, respectively. In addition, the ratio of ΔNH4+-N, ΔNO2--N and ΔNO3--N was basically 1 ∶1 ∶0.18. Critical flux of the Amx IMBR was 8.63 L/(m2·h),which was consistent with the long-term operational results. Tightly bound extracellular polymeric substances were the key factors to membrane fouling, in which the content of protein and polysaccharide were 2.42 g/m2 and 0.84 g/m2, respectively. During the operation, morphology of the Anammox bacteria changed from red granular sludge to planktonic sludge, which was one of the most significant characteristics of the Amx IMBR. High-throughput sequencing results indicated that the dominant microbial species of the Amx IMBR were Bacteroidetes (47.83%), Planctomycetes (21.2%) and Proteobacteria (18.85%). Among them, the dominant Anammox functional bacterium was Candidatus Kuenenia with relative abundance of 17.08%.
相似文獻(xiàn)/References:
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